Prerequisites:BIO 200 or BIO 325 or CHEM 301 or consent of instructor Requirement Fulfillment:Fulfills a course requirement in the Biology Core Concentration Delivery: Laboratory-intensive course. Students acquire the core set of skills involved in cloning specific genes. Techniques include the purification of DNA and RNA molecules; analysis of their size by gel electrophoresis; enzymatic amplification of DNA by PCR; construction of recombinant DNA molecules; detection of specific genes by Southern hybridization and DNA sequencing. Application of these techniques to the areas of evolutionary biology, forensics, and medicine are illustrated.
